Structure and methodology

Overview

The project is organized into four interconnected Work Packages (WPs) covering management, data generation, genomic analysis, and dissemination.

WP1 – Management and Scientific Coordination (Lead: University of Ferrara)

Ensures overall coordination, project monitoring, and communication between research units through regular meetings and progress evaluations.

WP2 – Pangenome Graph Development (Leads: University of Ferrara & University of Padua)

• Selection of 16 wild and landrace accessions from the Mediterranean region.

• Generation of high-quality chromosome-level genome assemblies using PacBio HiFi sequencing and Hi-C scaffolding.

• Integration of the assemblies into a comprehensive pangenome graph that will serve as a reference structure for future research.

WP3 – Investigating White Lupin Domestication (Leads: University of Ferrara & Marche Polytechnic University)

• Variant discovery across ~350 resequenced genomes.

• Population structure and demographic reconstruction to infer domestication events.

• Identification of genomic regions under artificial selection and candidate loci relevant for breeding.

WP4 – Promotion and Dissemination (Lead: University of Ferrara)

• Open-access publication of results and genomic data (NCBI, GenBank).

• Organization of workshops for young researchers on pangenomics and legume evolution.

• Outreach to farmers, breeders, and the public through events, social media, and collaborations with the EU INCREASE project.

Expected Outcomes

• A publicly available pangenome reference for Lupinus albus.

• New insights into the genetic basis of domestication.

• Strengthened collaboration among Italian and European research institutions